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KMID : 0370219960400010072
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Yakhak Hoeji
1996 Volume.40 No. 1 p.72 ~ p.77
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Characteristics of Enzyme Sensors using Carboxylated PVC for Immobilizing Penicillinase
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김기묘/Kim KM
김영학/이은엽/허문회/안문규/Kim YH/Lee EY/Hur MH/Ahn MK
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Abstract
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Penicillin sensor was manufactured by immobillizing penicillinase with glutaraldehyde on the H+-selective membrane based on PVC-COOH-TDDA. This membrane was not interfered by K+ ion in Pc-G potassium salt. When enzyme was immobilized with glutaraldehyde, the PVC-COOH matrix was more effective than PVC matrix. Calibration curve calculated from Nernst equation was not linear. But potential was relative to concentration of Pc-G. And maximal potentiometric velocity was also relative to concentration of Pc-G. Therefore, it may be applied to Michaelis-Menten equation. The penicillin sensor was useful for determination of Pc-G at concentration of 0.1~10mM level.
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KEYWORD
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